- Sylvester (Looney Tunes) as Tom
- Tweety Bird (Looney Tunes) as Jerry
- Anna (Frozen) as Tom's Owner
- Flynn Rider (Tangled) as the Moving Man
- Alpha (Up) as Bulldog
- Alex (Madagascar) as Pugsy
- Marty (Madagascar) as Frankie de Flea
- The Stabbington Brothers (Tangled) as the Straycatchers
- Vultures (Ice Age: The Meltdown) as Alley Cats
- Violet Parr (The Incredibles) as Robyn Starling
- Captain Chantel DuBois (Madagascar 3: Europe's Most Wanted) as Aunt Figg
- Gru (Despicable Me) as the Patrolman
- Hans (Frozen) as Lickboot
- Scud (Toy Story) as Ferdinand
- Ian Hawke (Alvin and the Chipmunks) as Dr. Applecheek
- Horton (Horton Hears a Who!) as Droopy Dog
- Bob Parr/Mr. Incredible (The Incredibles) as Daddy Starling
- Kristoff (Frozen) as Captain Kiddie
- Blu (Rio) as Squawk
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Sylvester and Tweety: The Movie
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