- Link (from Zelda) as Captain Neweyes
- Young Link (from Zelda) as Vorb
- Shadow as Stubbs the Clown
- Dr. Eggman as Professor Screweyes
- Toad (from Mario) as Buster the Baby Bird
- Peach (from Mario) as Buster's Mother
- Mario (from Mario) as Buster's Father
- Toadette, Baby Mario, Baby Luigi (from Mario) as Buster's Siblings
- Vanilla as Sasha's Mother
- Ganondorf (from Zelda) as Rex is a real animal
- Frog Naveen and Frog Tiana as Louie and Cecilia is a chimpanzees
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